DNA purification from multiple sources in plant research with homemade silica resins.

Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format.

For DNA purification to be functionally integrated into the microchip for high-throughput DNA analysis, a miniaturized purification process must be developed that can be easily adapted to the microchip format. In this study, we evaluate the effectiveness of a variety of silica resins for miniaturized DNA purification and gauge the potential usefulness for on-chip solid-phase extraction. A micro...

Recycling of anion-exchange resins for plasmid DNA purification.

Plasmid DNA of high purity is critical for molecular biology studies such as subcloning, DNA sequencing, vector construction and expression and functional analysis. The classical method of plasmid DNA purification using cesium chloride/ethidium bromide buoyant-density centrifugation is generally time-consuming and expensive and has gradually been replaced by the use of silica-based, anion-excha...

معرفی گیاه دارویی دارمازو (Quercus infectoria Oliv)در کوههای زاگرس و تعیین DNA بارکدینگ آن

Background: Quercus infectoria is one of the most important medicinal plants in the Zagros mountains, which from the ancient time has been taken into consideration as a known medicinal plant. Studies have shown, this species contains a wide range of medicinal properties. In this research comprehensive introduction of this medicinal tree, places of distribution and manners of correct diagnosis ...

Trace contamination following reuse of anion-exchange DNA purification resins.

Purification and subunit structure of deoxyribonucleic acid-dependent ribonucleic acid polymerase I from the mouse myeloma, MOPC 315.

DNA-dependent RNA polymerase I was purified from the mouse myeloma, MOPC 315. The enzyme was solubilized from purified nucleoli and subjected to chromatography on CM-Sephadex, DEAE-Sephadex, and phosphocellulose ion exchange resins and to sedimentation in sucrose gradients. These procedures yielded a chromatographically homogeneous enzyme which was purified almost lO,OOO-fold with respect to wh...